小细胞肺癌外周血和组织中耐药基因表达检测

《小细胞肺癌外周血和组织中耐药基因表达检测》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、者外周血淋巴细胞MDRlmRNA和MRPmRNA的表达水平增强；免疫组化结果显示P．gP，MRP，TOPO-1I在SCLC中均有不同水平表达，它们的表达对SCLC的耐药起重要作用，因此检测这三种耐药基因有助于判断化疗疗效及预后；在无法获得SCLC肿瘤组织情况下，通过抽取外周静脉血检测MDRlmRNA和MRPmRNA的表达来预测化疗疗效是可行的。硕士研究生傅兴宁(病理学)指导教师陈桦主任医师关键词：小细胞肺癌；外周血；耐药基因；逆转录聚合酶链反应法；免疫组织化学技术Detectionoftheexpressionofre

2、sistancegenesinsmallcelllungcancerAbstractSmallcelllungcancer(SCLC)accountsfor15％-20％ofalllungcancers，whichoriginsfromtheneuroendocrinefeaturesofendodermcells．Itisahighlymalignanttumor,withthecharacteristicsofrapidgrowthandearlymetastasis．Inadditiontolittleearly

3、SCLC，chemotherapyandradiotherapywereusuallyinvolvedinmedicaltreatments．Inrecentyears，despitegreatprogressesachievedincombinedtreatmentmeasuresincludingchangesofdrugcombination，drugdosesortimeofoperation，clinicaltherapeuticeffectisstillnotsatisfactory．Multidrugre

4、sistance(MDR)isthemainreasonresponsibleforthefailureofchemotherapyandreplaseoftheSCLC．SomestudiessuggestMDRphenomemonintumorcellsiscloselyrelatedwithoverexpressionofMDR1andMRPgenes．However,idealreversaldrugstargettingforMDRisstillunderresearch．objeet：Todetermine

5、thesignificanceofMDR1gene、MRPgeneTOPO一11geneexpressioninperipheralbloodlymphocytes(PBMCs)ofSCLCpatientsbeforeandafterchemotherapy，andexploretheproteinlevelofP—gP、MRP、TOPO—IIinSCLCtumortissues，aswellasstudythecorrelationofMDRl、MRP、andTOPO—IIexpressioninPBMCsandtu

6、mortissues．Methods：RT-PCRmethodandimmunohistochemistrymethodwereusedinthepresentstudy．Results：1．Detectingthepherepheralbood，theexpressionlevelsoftheMDRlmRNAandMRPmRNAinPBMCsinSCLCgroupwerehi曲erthanthatinthecontrolgroup(P<0．05)．TheexpressionlevelsofMDR1mRNAandMRP

7、mRNAafterchemotherapyinSCLCweresignificantlyhigherthanbeforechemotherapy(P<0．05，O．001)．2．TheexpressionlevelsofMDR1mRNAandMRPmRNAinextensivestageweresignificantlyhigherthaninlimitedstageOfSCLCafterthreecyclesofchemotherapyfP<0．05)．Nocorrelationswerefoundbetweenth

8、eexpressionlevelsofMDR1mRNAandMRPmRNAinPBMCsandage，gender，smokinghistory,tumorsize，clinicalstagesandlymphnodemetastasis(P>0．05)．3．Intumortissues．thepositiveratesofP-g