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ID:33382944
大小:2.31 MB
页数:43页
时间:2019-02-25
《igf-1r和igf1在胃肠道间质瘤中的表达及意义》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、郑州大学硕士学位论文IGF--1R和IGF1在胃肠道间质瘤中的表达及意义姓名:郭大鹏申请学位级别:硕士专业:外科学指导教师:张谢夫201204摘要IGF.1R和IGFl在胃肠道间质瘤中的表达及意义研究生郭大鹏导师张谢夫郑州大学第一附属医院胃肠外科中国郑州450052目的探讨胰岛素样生长因子1受体(insulin-likegrowthfactorreceptor,IGF.1R)和胰岛素样生长因子l(insulin.1ikegrowthfactor,IGFl)在胃肠道间质瘤(gastrointestinalstromaltumo
2、rs,GISTs)发病机制中的作用及临床意义。方法应用RT-PCR法检测IGF.1R和IGFl在40例胃肠道间质瘤和对照组中mRNA的表达水平,并同CDll7在GISTs中表达进行对比分析。结果在胃肠道间质瘤中,IGF.1R和IGFl呈高表达,明显高于肿瘤旁组织俨<0.05),在5例CDll7阴性表达的GIST中,有4例(80.0%)IGF。1R呈阳性表达,有2例(40.0%)IGFl呈阳性表达;IGF.1R和IGFl的表达与性别、年龄、组织类型不相关,而与NIH分级(肿瘤大小、核分裂数)、肿瘤粘膜受侵和肿瘤侵袭转移相关伊<
3、0.05)。结论IGF.1R和IGFl在胃肠道间质瘤的发生发展中起到重要作用,尤其在CDll7阴性的GIST中IGF.1R表达率较高,联合检测用于GIST的诊断具有较好的互补性,并可能参与了肿瘤的侵袭转移。关键词:胃肠道问质瘤:IGF.1R;IGFl;CDl17;RT-PCRAbstractTheExpressionandSignificanceofIGF-1RandIGFlingastrointestinalstromaltumorsByDapengGuoSupervisorProf.XiefuZhangGastroint
4、estinalSurgery,TheFirstaffiliatedHospitalofZhengzhouUniversity,Zhengzhou450052,ChinaObjectiveToinvestigatetheeffectionsofIGF-1RandIGFIingastrointestinals仃omaltumors’pathogenesisandtheirclinicalsignificance.MethodImPCRWasusedtOdeterminetheIGF.1RandI(强1mRNAexpression
5、sin40casesofgastrointestinalstromaltumorstissuesandthenormaltissues.Bycomparison,theexpressionofCDl7ingastrointestinalstromaltumorswasanalyzed.ResultIngastrointestinalstromalUlmors,theexpressionsofIGF-1RandIGFlweresignificantlyhigherthanthoseofnormalfissues(P<0.05)
6、;In5caseswhichCDll7Wasnegative,theexpressionrateofIGF一1RandIGFlWas80.O%,40%respectively;TheexpressionofIGF·1RandIGF1WascorrelatedwiththeNIHstage(tumorsize,mitoticfigures),invasionofmucosa,tumorinvasionandmetastasis(P<0.05).Theexpressionhadnorelationshipwithgender,a
7、ge,differentiatetissuetype.ConclusionIGF-1RandIGFlplayanimportantroleinthegenesisanddevelopmentofgastrointestinalstromaltumors.EspicallyintheGISTofCDI17negative,IGF·IRshowsmorepositive,combine谢血CDl17hasagoodcomplementarityforthediagnosisofGIST.IGF一1RandIGF1mayassoc
8、iatedwiththepresenceofinvasionandmetastasisingastrointestinalstromaltumors.Keywordsgastrointestinalstromaltumors;IGF-1R;IGFl;CDll7;RT-PCR前言IGF.1R
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