中国大枣资源化学的研究

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1、obtainedfromthisplantforthefirsttime．Inthethirdchapter，anHPLC．DADmethodconjugatedwithchemometficsapproachwausestablishedandusedtoevaluatethequalityoffructusjujubae．Firstly，asimpleandsensitiveHPLC．DADmethodhasbeendevelopedforthefirsttimetosimultaneouslydeterminetentriterpe

2、noidacids(ceanothicacid，alphitolicacid，zizyberanalacid，zizyberanalicacid，epiceanothicacid，ceanothenicacid，betulinicacid，oleanolicacid，ursonicacidandzizyberenalicacid)infructusjujubae．ThisHPLCassayWasperformedonareversed．phaseC18column(250mmx4．6rain，5岬)withthecolunmtempera

3、tureat35。C．ThemobilephasewascomposedofA(acetonitfile)andB(O．05％aqueousphosphoricacid，v／v)．TheflowrateWas1．0mL／minandthedetectionwavelengthWassetat205hidforreferencecompounds1-9and238halforreferencecompound10．Allcalibrationcurvesshowedgoodlinearregression舻>0．9999)withinthe

4、testrange．Theestablishedmethodshowedgoodprecisionandaccuracywithoverallintra—dayandinter-dayvariationsof0．43—1．72％and0．53—2．45％，respectively，andoverallrecoveriesof94．98-104．09％fortlletencompoundsanalyzed．Thevalidatedmethodwassuccessfullyappliedfor也esimultaneousdeterminati

5、onofthe10triterpenoidacidsin42batchesoffructusjujubaewllichcontajned36cultivarsfrom22cultivationregions，andwereinvestigatedandauthenticatedasZjujuba．Secondly．hierarchicalclusteringanalysis(HCA)andprincipalcomponentsanalysis(PEA)wereperformedtodifferentiateandclassifythesa

6、mplesbasedontllecontentsofthetenldterpenoidacids．TheresultsshowedthatthetotalcontentofthetentilterpenoidacidsinthecultivarofDongzao(CultivaredinZhanhua,ShandongProvince)wastripleofothersamples，whichsuggestedthatDongzaoisapotentialcultivarofZjujubaconsideringthecontentoftr

7、iterpenoidacids．Besides，theresultsofHCAandPCAshowedthatthediversityofDongzaoWasmostobvioustotheothercultivarsofZjujubaint11e36testedcultivars．ThepresentedHPLC．DADmethodconjugatedwithchemometricsapproachWasdemonstratedtobeveryhelpfulinusingfructusjujubaeresources，andWaspos

8、siblyusefulinchemotaxonomiccharacterization．Inthefourthchapter,thequalitystandardoffructusjujuba