dna分子标记技术在黄颡鱼遗传学研究中的应用

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1、JournalofNanchangInstituteofTechnologyISSN1674—-007614／14PP72-78Voloume30，Number1，February2011ApplicationofDNAmolecularmarkersingeneticresearchofPt'elteobagrusMAOHuiling，XIAOHui，GEXinqi，OUYANGLian(SchoolofLifeScienceandFoodSafety，NanchangUniversity，Nanchang330031，China)Abstr

2、act：TheapplicationofDNAmolecularmarkersingeneticresearchofPelteobagruswasintroducedinthepaper．Itin—volvesgermplasmidentification，phylogeneticevolution，andgeneticdiversity．Furthermore，theexistingproblemsandprospectofDNAmolecularmarkerapplicationwasanalyzedindetail．Keywords：DN

3、Amolecularmarker；Pelteobagrus；geneticdiversity1Background1．1GeneralintroductionofPelteobagrusGenusPeheobagrusbelongstoOsteichtliyes，Siluriofmres，Bagrida[Ij．Asakindofsmallbutfamouseconomicfish，Peheobagrusisgentleomnivoresandhasaremarkableabilitytoadapttoecologicalenvironment．

4、Piswellacceptedbyconsumersbecauseofitstendermeat，richnutrition；highrateoffleshandconvenientcooking：Thus，PeheobagrushasbecomeanimportanteconomicfishspeciesinChina．1．2IntroductiononmolecularnlarkersMostoftheearlyresearchonPeheobagrusfocusedonartificialculturetechnologyanddisea

5、secontrol，fromsuchperspectivesasbiology，physiology，biochemistry，etc．WiththedevelopmentoftheDNAmolecularmarker，thistechnologypropelledthestudiesonPeheobagrus．MoreandmoreresearchersbegantostudyPel~obagrusbyusingDNAmolecularmarkers．DNAmolecularmarkerisakindofgeneticmarkersbased

6、ontheDNApolymorphism．Itcaninheritstablywithsimpleinheritancemodeandreflectsbiologicalcharacteristicsofindividualandpopulation．ComparedwithMorpho—logicalMarkers，CytologicalMarkersandBiochemicalMarkers，DNAmolecularmarkeriswidelyusedsinceitap—peared．Itsadventageslieinitsmultipl

7、etaggedsites，highspecificityandreproducibility，stablegeneticmarker．Thosegreatamountsofinformationwouldnotbeinfluencedbyorganism’sage，developmentalstage，genderorfeed—ingenvironments．DNAmolecularmarkerwouldbecomemoreandmoreimportantinstudyonaquaticanimal，faeili-tatedbytherapid

8、developmentofPCRandelectrophoresistechnology．Soitiscurrentlywidelyappliedin