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1、1752RegularArticleBiol.Pharm.Bull.35(10)1752–1760(2012)Vol.35,No.10ARatModelforStableChronicObstructivePulmonaryDiseaseInducedbyCigaretteSmokeInhalationandRepetitiveBacterialInfectionaa,a,bbbbbYaLi,Su-YunLi,Jian-ShengLi,*LiDeng,Yan-GeTian,Su-LiJiang,YingWang,andaYu
2、an-YuanWangaInstituteofRespiratoryDiseaseandCentreLaboratory,TheFirstAffiliatedHospitalofHenanUniversityofbTraditionalChineseMedicine;andInstituteofGerontology,HenanUniversityofTraditionalChineseMedicine;ZhengzhouCity,HenanProvince450008,China.ReceivedMay4,2012;acce
3、ptedJuly9,2012;advancepublicationreleasedonlineAugust3,2012Todevelopastablechronicobstructivepulmonarydisease(COPD)modelinrats.Sprague-Dawleyratsweretreatedwithcigarette-smokeinhalation(CSI)for12weeks,repetitivebacterialinfection(RBI)for8weeks,orthecombinationofthe
4、two(CCR)for12weeksandfollowedupfortheadditional20weeks.Tidalvolume(VT),peakexpiratoryflow(PEF)and50%VTexpiratoryflow(EF50),histologicalchangesinthelungs,andlevelsofthecytokinestumornecrosisfactor(TNF)-α,interleukin(IL)-8,andIL-10inserumandbronchialalveolarlavagefluid(
5、BALF)wereexaminedatintervalsduringthe32weekstudyperiod.Therightventricularhypertrophyindex(RVHI)wasalsodeterminedatthesametimes.VT,PEF,andEF50weredecreasedinratswithCOPDcomparedtothecontrol.TheexpressionofTNF-α,IL-8andIL-10increasedinbothserumandBALFwithasimilartre
6、nd.BronchioleandarteriolewallthicknessandthedegreeofbronchiolestenosisandalveolarsizeincreasedinCOPDrats.RVHIwasreducedgraduallyfollowingthetreatment.AllofthesechangesweremorepronouncedintheCCR-treatmentgroupthanintheothergroups.OurresultshaveshownthatCSIorRBIalone
7、caninduceCOPDinrats,butthatthecombinationofCSIwithRBIinducesastableCOPDthathasmoresimilaritytocomplicationsseeninpatientswithCOPD.ThiscombinationmaythereforeprovideamoreappropriatemodelforstudyofhumanCOPD.Keywordspulmonarydisease;chronicobstructive;animalmodel;pulm
8、onaryfunction;histologicalchange;rightventricularhypertrophyindexChronicobstructivepulmonarydisease(COPD)hasbeencausedbybacterialorviralinfection
