小麦玉米转基因植株的抗性鉴定

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1、目录中文摘要1英文摘要21引言31.1植物PGIP概况31.2转基因植株的鉴定方法的研究进展31.2.1标记基因的使用31.2.1.1选择标记基因31.2.1.2报告基因41.2.2分子检测41.2.2.1分子标记51.2.2.2分子杂交51.2.3免疫技术61.2.3.1酶联免疫法(ELISA)61.2.3.2免疫荧光技术61.3.小麦赤霉病、玉米纹枯病真菌病害人工接种方法的研究进展71.3.1小麦赤霉病人工接种方法71.3.2玉米纹枯病人工接种方法71.4本研究的目的和意义82材料与方法82.1实验材料82.1.1

2、目的基因82.1.2植物材料82.1.3菌株和载体92.2实验方法92.2.1转基因植株的鉴定92.2.1.1转化株的PCR检测9221.1.1扌专基因植株DNA的微量提取(CTAB法)92.2.1.1.2转基因植株的PCR鉴定92.2.2转化株的PPT涂叶检测10223真菌病害抗性鉴定102.2.3.1小麦赤霉病抗性鉴定102.2.3.2玉米纹枯病抗性鉴定101.2.3.1结果与分析113」转化株的PCR检测113.2转化株的PPT涂叶检测113.3转化株的真菌抗病性鉴定123.3.1小麦赤霉病的抗性鉴定1123.3

3、.2玉米纹枯病的抗性鉴定131.2.3.2讨论151.2.3.3结论16参考文献17致谢18ContentsAbstract1Englishabstract23..Introduction33PlantPGIPProfile33Progressinidentificationoftransgenicplants33.2Theuseofmarkergenes33.2.1Selectablemarkergene3223.2Reportergene4122Moleculardetection41.2.2・1Molecular

4、markers53.3.2Molecularhybridization5123Immunoassaytechnology6(1)ELISA61-2.3.2Immunofluorescencetechnique62.1wheatscabblotchfungaldiseasesofcomsheathblightartificialinoculationmethod7・3・1WheatFusariumheadblightartificialinoculationmethods7Maizesheathblightartific

5、ialinoculationmethods7Thepurposeandsignificanceofthisstudy8Materialsandmethods8Experimentalmaterials8Thetargetgene8Plantmaterial8Strainsandvector92.2Experimentalmethods9221Identificationoftransgenicplants9PCRdetectionoftransformingstrains9Switchtotransgenicplant

6、sDNAExtraction(CTAB)9PCRidentificationoftransgenicplants9TransformingstrainsPPTcoatingsolutiondetection10223Identificationoffimgaldiseaseresistance102.23JWheatscabresistanceidentification10Identificationofcomsheathblightresistance10ResultsandAnalysis11PCRidentif

7、icationoftransgenicplants11TransformingstrainsPPTcoatingsolutiondetection11Identificationoffungaldiseaseresistance12331Wheatscabresistanceidentification12Identificationofcomsheathblightresistance13Discuss15Conclusion16References13Acknowledgement17小麦玉米转基因植株的抗性鉴定摘

8、要：多聚半乳糖醛酸酶抑制蛋白(polygalacturonaseinhibitingprotein,PG1P)是一种富含亮氨酸重复的细胞壁结合蛋口。它是一种特界性蛋口，能够非竟争性地抑制病原菌的内切多聚半乳糖醛酸酶(endo・polygalacturonase,endo-PG)活性，增强植物的抗病性。当植物受到病原菌、机械创伤、热