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1、BiochemicalSystematicsandEcology38(2010)993–999ContentslistsavailableatScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoEstimationofgeneticvariationinCynodondactylonaccessionsusingtheISSRtechniqueChun-QiongHuang,Guo-DaoLiu*,Chang-j
2、unBai,Wen-qiangWang,Shao-yunZhou,Dao-QengYuTropicalCropsGeneticResourcesInstitute,ChineseAcademyofTropicalAgriculturalSciences/KeyLaboratoyofTropicalCropsGermplasmResourcesUtilization,MinistryofAgriculture,Danzhou571737,PRChinaarticleinfoabstractArticlehistory:Geneticvariationin5
3、5accessionsofCynodondactylonwasestimatedusinginter-simpleReceived1September2010sequencerepeat(ISSR)markers.Theplantmaterialsusedinthisstudyoriginatedfrom17Accepted26September2010countries.Atotalof236ISSRfragmentsweregeneratedwith14primers.FragmentsizesAvailableonline9November2010
4、rangedfrom200to3000bp.Allscorablebandswerepolymorphicinnatureandnoneoftheprimersusedproducedmonomorphicbands,indicatingahighlevelofgeneticvaria-Keywords:tioninthisgrass.TheaccessionswerefoundtobeclusteredintoeightmajorgroupsCynodondactylonthroughtheunweightedpair-groupmethodwitha
5、rithmeticaverages.GeneticsimilarityGeneticvariationcoefficients(GSC)amongthe55accessionsrangedfrom0.52to0.95.TheresultsclearlyISSRmarkerindicatethatahighlevelofvariationexistsinCynodonaccessions.ThisstudyshowsthattheISSRtechniqueisareliabletoolfordifferentiatingCynodonaccessionsan
6、dfordeter-miningthegeneticrelationshipsamongthem.Ó2010PublishedbyElsevierLtd.1.IntroductionThegenusCynodon,whichcomprises9speciesand10varieties,originatedfromtropicalorsubtropicalareas(Taliaferro,1995).Cynodondactylon(commonbermudagrass),thelargestspeciesinthegenus,iswidelydistri
7、butedgeographicallyandisoneofthemostcommonlyusedwarmseasonturfgrassforturfestablishmentofpublicgreenland,golfcourses,andsportsfieldsinmanycountries(AckersonandYoungner,1975;Dudecketal.,1983).Asahighqualityturfgrass,bermudagrassischaracterizedbyitsgoodtexture,highsoddensity,highwea
8、r,goodcolor,strongdroughtresistance,ands
