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1、BiochemicalSystematicsandEcology46(2013)108–115ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversityanalysisofCynodondactylon(bermudagrass)accessionsandcultivarsfromdifferentcountriesbasedonISSRa
2、ndSSRmarkersa,b,ccdaaa,*ZhiyongWang,LiLiao,XuejunYuan,HailinGuo,AiguiGuo,JianxiuLiuaInstituteofBotany,JiangsuProvinceandChineseAcademyofSciences,Zhongshanmenwai,Nanjing210014,ChinabKeyLaboratoryofProtectionandDevelopmentUtilizationofTropicalCropGermplasmResources,HainanUniversity,Ministr
3、yofEducation,Haikou570228,ChinacCollegeofAgronomy,HainanUniversity,Haikou570228,ChinadCollegeofLifeScience,QiongzhouUniversity,Sanya572000,ChinaarticleinfoabstractArticlehistory:ISSRandSSRmarkerswereusedtoevaluategeneticdiversityamong33CynodondactylonReceived18May2012accessionsand22culti
4、varsfromfourdifferentcountriesinordertoprovideinformationAccepted9September2012onhowtoimprovetheutilizationofbermudagrassgermplasms.EightyeightbandswereAvailableonline16October2012amplifiedbynineSSRprimercombinationsand236bandswereobservedfrom23ISSRprimers.Theresultsshowedthat97.7%oftheSS
5、Rprimersand86.9%oftheISSRprimersKeywords:werepolymorphic.Thegeneticsimilaritycoefficients(GSC),genediversity(He)andCynodondactylonvar.dactylonShannonindex(I)were0.58–0.97,0.27and0.41,respectively,forISSRand0.52–0.97,0.29,Geneticdiversityand0.43forSSR.TheUPGMAanalysisclusteredthe55accessio
6、ns(cultivars)intothreeGermplasmISSRmarkergroups.TheclusterresultsproducedbytheISSRdatawereclosetotheSSRdataresults.SSRmarkerAnalysisbasedonthecombinedISSRandSSRdatawasmorecloselyrelatedtothegeographicaldistributionofthetestedgermplasm.Ó2012ElsevierLtd.Allrightsreserved.1.IntroductionTher
7、earetenspeciesandninevarietiesofCynodonintheworldandtwospeciesandonevarietyarefoundinChina.Cynodondactylonvar.dactylon(commonbermudagrass)isthemostimportantCynodonspeciesandisgeographicallywidelydistributedbetweenlatitudesof45Nand45S,penetratingtoapproximately53NinEuro
