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1、BiochemicalSystematicsandEcology37(2009)549–555ContentslistsavailableatScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoAnalysisofgeneticvariationingrasscarp(Ctenopharyngodonidellus)fromnativeandcolonizedreg
2、ionsusingISSRmarkersaa,*baaQinChen,Cheng-HuiWang,GuoqingLu,XiaoSong,Jia-WeiXu,aaQin-LingYang,Si-FaLiaKeyLaboratoryofAquaticGeneticResourcesandUtilization,MinistryofAgriculture,ShanghaiOceanUniversity,Shanghai201306,ChinabDepartmentofBiology,UniversityofNeb
3、raskaatOmaha,Omaha,NE68182,USAarticleinfoabstractArticlehistory:Thegrasscarp(Ctenopharyngodonidella),afreshwaterspeciesnativetoChina,havebeenReceived15May2009introducedinmorethan100countries.Inthispaper,intersimplesequencerepeat(ISSR)Accepted22August2009ma
4、rkerswereusedtoevaluategeneticvariationwithinandamonggrasscarppopulationssampledfromitsnative(China)andcolonizedhabitats(USA,Hungary,andJapan).Twenty-Keywords:twoISSRprimerswereusedtogenerate207bands,ofwhich82(39.61%)wereCtenopharyngodonidellapolymorphic.T
5、hehighestgeneticdiversitywasobservedintheYangtzeRiverpopulation,GeneticdiversitywhereasthelowestdiversitywasfoundintheToneRiverpopulation(Japan).ComparedDifferentiationwithcolonizedpopulations,thenativepopulationspossessapproximatelytwiceasmuchISSRgeneticd
6、iversity.TheAMOVAanalysisshowedarelativelyhighlevelofgeneticvariationwithinpopulations.Significantgeneticdifferenceswererevealedbothamongthenativepopulationsandbetweenpoolednativeandcolonizedpopulations.Ó2009ElsevierLtd.Allrightsreserved.1.IntroductionThegr
7、asscarp(Ctenopharyngodonidellus)isafreshwaterspeciesnativemainlytoChina(e.g.,theYangtzeR.,theAmurR.andthePearlR.)(LiandFang,1990;Lietal.,1998).Itplaysanimportantroleinaquaculture,withitstotalproductionbeing4.01milliontonsin2006,thesecondhighestinworldfishpr
8、oduction(FAO,2006).Sincethegrasscarpisoneofthemostimportantfreshwaterspecies,itsgeneticvariationanddiversityhavebeenextensivelystudiedusingvariousgeneticmarkerssuchasisoenzymes(WuandWang,1987a,b,1992;ZhaoandL
