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1、J.Eukaryot.Microbiol.,53(5),2006pp.358–363r2006TheAuthor(s)Journalcompilationr2006bytheInternationalSocietyofProtistologistsDOI:10.1111/j.1550-7408.2006.00113.xPopulationGeneticStructureofCarchesiumpolypinum(Ciliophora:Peritrichia)inFourChineseLakesInferredfromISSRFingerprinting:HighDiversitybutLow
2、Differentiationa,b,ca,caaaWEN-JINGZHANG,JUNYANG,YU-HEYU,SHAO-WUSHUandYUN-FENSHENaStateKeyLaboratoryofFreshwaterEcologyandBiotechnology,InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China,andbSchoolofEnvironmentalStudies,ChinaUniversityofGeosciences,Wuhan430074,China,andcGraduateSchoo
3、loftheChineseAcademyofSciences,Beijing100039,ChinaABSTRACT.AlthoughtheperitrichousciliateCarchesiumpolypinumiscommoninfreshwater,itspopulationgeneticstructureislargelyunknown.Weusedinter-simplesequencerepeat(ISSR)fingerprintingtoanalyzethegeneticstructureof48differentisolatesofthespeciesfromfourlake
4、sinWuhan,centralChina.Usingeightpolymorphicprimers,81discernibleDNAfragmentsweredetected,amongwhich76(93.83%)werepolymorphic,indicatinghighgeneticdiversityattheisolatelevel.Further,Nei’sgenediversity(h)andShannon’sInformationindex(I)betweenthedifferentisolatesbothrevealedaremarkablegeneticdiversity
5、,higherthanpreviouslyin-dicatedbytheirmorphology.Atthesametime,substantialgeneflowwasfound.Sothemainfactorsresponsibleforthehighlevelofdiversitywithinpopulationsareprobablyduetoconjugation(sexualreproduction)andwidedistributionofswarmers.Analysisofmo-lecularvariance(AMOVA)showedthattherewaslowgeneti
6、cdifferentiationamongthefourpopulationsprobablyduetocommonancestryandfloodingevents.Theclusteranalysisandprincipalcomponentanalysis(PCA)suggestedthatgenotypesisolatedfromthesamelakedisplayedahighergeneticsimilaritythanthosefromdifferentlakes.BothanalysesseparatedC.polypinumisolatesintosub-groupsacco
7、rdingtothegeographicallocations.However,thereisonlyaweakpositivecorrelationbetweenthegeneticdistanceandgeographicaldistance,suggestingaminoreffectofgeographicaldistanceonthedistributionofgeneticdiversitybet
