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1、BiochemicalSystematicsandEcology42(2012)35–48ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoAssessmentofgeneticdiversityandstructureofImperialCrown(FritillariaimperialisL.)popu
2、lationsintheZagrosregionofIranusingAFLP,ISSRandRAPDmarkersandimplicationsforitsconservationa,ba,c,*aaSajadBadfar-Chaleshtori,BehrouzShiran,MasoomehKohgard,HassanMommeni,aaaaAkramHafizi,MahmoodKhodambashi,NedaMirakhorli,KarimSorkhehaDepartmentofPlantBreedingand
3、Biotechnology,FacultyofAgriculture,ShahrekordUniversity,Shahrekord,POBox115,IranbDepartmentofAgronomyandPlantBiotechnology,FacultyofAgriculture,PayameNor-e-TehranUniversity,Tehran,POBox,IrancInstituteofBiotechnology,ShahrekordUniversity,Shahrekord,POBox115,Ir
4、anarticleinfoabstractArticlehistory:FritillariaimperialisgrowsathighaltitudesofthealpineZagrosregionofIranandhasReceived2August2011ahighmedicinalandornamentalvalueandimportance.InthisstudyAFLP,ISSRandRAPDAccepted28December2011markerswereusedtodetectintra-andi
5、nter-populationgeneticdiversityofF.imperialis.Availableonline9March2012140genotypes,belongingtosevenpopulations,werestudiedusingsixrandomprimers,sixISSRprimersandfiveAFLPprimercombinations,toinvestigatethegeneticstructureKeywords:betweenpopulations.Percentageo
6、fpolymorphicloci,estimatedusingNei’sgeneticFritillariaimperialisdiversityindexandShannon’sinformationindexrevealedlowormoderatelevelsofGeneticdiversitygeneticvariationswithineachF.imperialispopulation.Similarly,themeancoefficientofMolecularmarkerAFLPgenediffer
7、entiationbetweenpopulations(Gst)forRAPD,ISSRandAFLPwere0.415,0.47ISSRand0.63respectively,indicatingthat58.5%,52.3%and36.1%ofgeneticdiversityresidedRAPDwithinthepopulation.AMOVAindicatedthatvariationamongpopulationswashigherthanthosewithinF.imperialispopulatio
8、ns.Geneflowwas0.70,0.54and0.29fromRAPD,ISSRandAFLPmarkeranalysisrespectivelyindicatingthattherewaslimitedgeneflowamongthepopulations.ResultsshowedthatAFLPisthebestmarkerforinvestigatingtheg
