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1、BiochemicalSystematicsandEcology48(2013)248–256ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversityofnaturalMiscanthussinensispopulationsinChinarevealedbyISSRmarkers111QiXiangZhang,Y
2、iKeShen,RuoXuanShao,JiaFang,YongQingHe,JunXiaRen,BingSongZheng*,GuoJuanChen*NurturingStationfortheStateKeyLaboratoryofSubtropicalSilviculture,ZhejiangA&FUniversity,Linan311300,ChinaarticleinfoabstractArticlehistory:MiscanthussinensisisatypicalperennialC4grassinAsia.Intheprese
3、ntstudy,ISSRReceived5September2012markerswereusedtoevaluatethegeneticvariationwithinandamonggrasspopulationsAccepted24December2012thatweresampledfromtheZhejiangandGuangdongProvincesofChina.BasedonnineAvailableonline12February2013ISSRprimers,206clearandreproducibleDNAfragments
4、weregenerated.Relativelylowlevelsofgeneticdiversityweredeterminedamongthepopulations.ThecoefficientofKeywords:geneticdifferentiationamongthepopulationswas0.52,whichindicatesthat52.3%oftheMiscanthussinensistotalmolecularvarianceexistsamongthepopulations.Suchahighlevelofdivergen
5、ceGeneticdiversitypresentamongthepopulationsmightbecausedbythecomplextopographyandvariableDifferentiationISSRclimaticconditionspresentinthetwoprovinces.AMOVArevealedthatthemajorityofthegeneticvariationwaswithinthepopulations(50.6%).Theapplicationofanovelmethod,whichcombinesge
6、ographicalcoordinatesandgeneticdifferentiationtodetectbarriersforgeneflow,allowedustoidentifytwozonesofloweredgeneflow.Ó2013ElsevierLtd.Allrightsreserved.1.IntroductionEnergycrisisandenvironmentaldegradationhaveledtothesearchforalarge-scalesubstitutionoffossilfuel(Ceotto,2008;B
7、enbiandBrar,2009).Manybotanistshavebeentryingtofindplant-basedbiofuelsinordertoreducetheuseoffossilfuel.Amongthem,Miscanthusisoneofthemostimportantbiofuelplantsthathastheadvantagesoffastgrowth,lowinput,andhighphotosynthesis(VisserandPignatelli,2001;Clifton-Brownetal.,2004;Hast
8、ingsetal.,2008),andiswidelyusedinEuropeasabioenergycrop.Miscanthusis
