genetic diversity within and among the wild populations of murraya

genetic diversity within and among the wild populations of murraya

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时间:2018-02-10

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1、BiochemicalSystematicsandEcology39(2011)139–144ContentslistsavailableatScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversitywithinandamongthewildpopulationsofMurrayakoenigii(L.)Spreng.,asrevea

2、ledbyISSRanalysisSushmaVerma,T.S.Rana*ConservationBiologyandMolecularTaxonomyLaboratory,NationalBotanicalResearchInstitute(CouncilofScientificandIndustrialResearch),RanaPratapMarg,Lucknow226001,IndiaarticleinfoabstractArticlehistory:Murrayakoenigii(L.)Sp

3、reng.,commonlyknownascurryleafplant,isfoundinthedifferentReceived10August2010hillyregionsofIndia.Inthepresentstudy,fifty-nineaccessionsrepresentingeightwildAccepted29January2011populationsofM.koenigiiwereanalyzedusingthirteenISSRprimers.Atotalof152bandsA

4、vailableonline22February2011wereamplified,outofwhich,136werepolymorphiccorrespondingto89.47%polymorphismacrosstheaccessions.ThepairwisepopulationgeneticdistanceswerecalculatedforalltheKeywords:populationsthatvariedfrom0.05to0.13betweenthepopulationsofM.k

5、oenigii.AMOVAandGeneticdiversityNei’sgeneticdiversityanalysisrevealedhighergeneticvariationswithinpopulationsthanPopulationstructureamongthepopulations.TheclusteringofpopulationsinthedendrogramwasnotinWildMurrayakoenigiiISSRcongruencewithgeographicalaffi

6、liations.TheresultsindicatethattheISSRmethodissufficientlyinformativeandpowerfultoestimatethegeneticdiversityinM.koenigiipop-ulations.AsM.koenigiiisanimportantwildplantgeneticresource,therefore,informationongeneticvariabilitymightbeapotentialsourceasbree

7、dingmaterialfordevelopmentofcommerciallyvaluabletraitsinM.koenigiiplants.Ó2011ElsevierLtd.Allrightsreserved.1.IntroductionThegenusMurrayaKoenigexL.,(Rutaceae)comprisesofabout11speciesofshrubsandsmalltreesdistributedintropicalandsub-tropicalregionsofSriL

8、anka,India,SouthChinatoSouth-EastAsia,Malaysia,NewGuinea,North-EastAustraliaandNewCaledonia(SwingleandReece,1967).InIndiathegenusisrepresentedbytwospecies,Murrayakoenigii(L.)Spreng.,andMurrayapaniculata(L.)Jack.,having(2n)18chrom

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