analysis of genetic diversity in acorus calamus populations in south

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1、BiochemicalSystematicsandEcology40(2012)156–161ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoAnalysisofgeneticdiversityinAcoruscalamuspopulationsinSouthandNorthEastIndiausingISSRmarkersaa,*aaaV.K.AbdulKareem,P.E.R

2、ajasekharan,B.S.Ravish,S.Mini,AnuradhaSane,bT.VasanthaKumaraDivisionofPlantGeneticResources,IndianInstituteofHorticulturalResearch,HessaraghattaLakeP.O.,Bangalore560089,IndiabSectionofMedicinalCrops,IndianInstituteofHorticulturalResearch,HessaraghattaLakeP.O.,Bangalore560089,Indiaarticleinfoabstr

3、actArticlehistory:Intersimplesequencerepeat(ISSR)markerswereusedtoestimategeneticdiversityReceived13April2011among21samplesofAcoruscalamus,anendangeredmedicinalherbfromSouthandNorthAccepted22September2011EastIndia.Outof100primersscreened14generateddistinctandreproducibleDNAAvailableonline11Novemb

4、er2011fragments.Usingtheseprimers,103discernibleDNAfragmentsweregeneratedofwhich53(51.5%)werepolymorphicandremaining50(48.5%)weremonomorphic.TheamplifiedKeywords:PCRfragmentsizerangedfrom450to2250bp.Nei’sgeneticdiversity(h)andShannon’sAcoruscalamusindex(I)amongthepopulationwereestimatedas0.2005and

5、0.2946respectively.ValueClonalplantISSRfortotalgenotypediversityamongpopulation(HT)was0.1893whilewithinpopulationGeneticdiversitydiversity(HS)wasfoundtobe0.0834.Themeancoefficientofgenedifferentiation(GST)Sweetflagwasashighas0.5595andthegeneflow(Nm)wasfoundtobe0.3937;bothindicatedrapidUPGMAgeneticdi

6、fferentiationamongthepopulationsofA.calamus.Analysisofmolecularvariance(AMOVA)indicatedthat43%wasattributabletoamong-populationsdiversityandtherest(57%)todifferenceswithinpopulations.TheUPGMAdendrogrambasedonWard’sminimumvariancealgorithmshowedahighlevelgeneticdivergencebetweenthepop-ulationsfrom

7、SouthandNorthEasternIndia(w119linkagedistance)whichexplainstheexistenceoftwogeographicallydistinctpopulations.Ó2011ElsevierLtd.Allrightsreserved.1.IntroductionAcoruscalamusL.(sweetflag,familyAcoraceae)isaneconomicallyim