genetic diversity of wild populations of tuberaria major (cistaceae), ann

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1、BiochemicalSystematicsandEcology45(2012)49–56ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversityofwildpopulationsofTuberariamajor(Cistaceae),anendangeredspeciesendemictotheAlgarveregion(Portugal),usin

2、gISSRmarkersa,*,1a,b,1bbHelenaTrindade,InêsSena,SandraGonçalves,AnabelaRomanoaUniversidadedeLisboa,FaculdadedeCiênciasdeLisboa,DepartamentodeBiologiaVegetal,InstitutodeBiotecnologiaeBioengenharia,CentrodeBiotecnologiaVegetal,C2,CampoGrande,1749-016Lisboa,PortugalbInstituteforBiotechnologyandBio

3、engineering,CentreofGenomicsandBiotechnology(IBB/CGB),FacultyofSciencesandTechnology,UniversityofAlgarve,CampusdeGambelas,Ed.8,8005-139Faro,PortugalarticleinfoabstractArticlehistory:Tuberariamajor(Willk.)P.Silva&Rozeira(Cistaceae)isanendangeredspeciesendemictoReceived30January2012theAlgarveregi

4、on(Portugal).Duetotheincreasingurbanpressure,naturalpopulationsAccepted28June2012arenowreducedtosmallnucleilocatedinthecenteroftheregion.GeneticvariationAvailableonline3August2012withinandamongthreepopulationsofthisspecieswasevaluatedusinginter-simplesequencerepeat(ISSR)markers.Atotalofeightpri

5、mersgenerated128welldefinedKeywords:bands,withanaverageof16bandsperprimer.Atthepopulationlevel,thepercentageofEndemicspeciespolymorphicbandsrangedfrom55to73%,whileatthespecieslevelthisvalueincreasedConservationimplicationsto98%.ThepopulationshowingthehighestgeneticvariabilitywasOlhãoandthelowest

6、TuberariamajorGeneticstructurewasGambelas.AccordingtotheclusteranalysisbasedonUPGMA,thepopulationGam-Geneticdiversitybelaswasclearlyseparatedfromtheothertwo,whichclusteredtogether.ThethreeISSRmarkerspopulationsrevealedahighlevelofgeneticdiversity,withaNei’sgeneticdiversityof0.197andShannon’sdiv

7、ersityindexof0.324.Ahighlevelofgeneflowwasobservedamongpopulations(Nm¼2.199),reflectingamoderatelevelofgeneticdifferentiation(GST¼0.185),andnocorrelationbetweengeneticandgeographicaldistances(r¼1.000;P>0.05).Basedontheresultsobtaine