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1、™METHODSINMOLECULARBIOLOGY•368DayDaySERIESEDITOR:JohnM.WalkerStaceyStacey™METHODSINMOLECULARBIOLOGY368CryopreservationandFreeze-DryingProtocols,SecondEditionCryopreservationandFreeze-DryingProtocols:EditedbyJohnG.DayCultureCollectionofAlgaeandProtozoa,ScottishAssociationofMarineScience,DunstaffnageM
2、arineLaboratory,Dunbeg,Argyll,UKCryopreservationCryopreservationandGlynN.StaceyDivisionofCellBiologyandImaging,NationalInstituteforBiologicalStandardsandControl,PottersBar,Herts,UKCryopreservationandFreeze-DryingProtocols,SecondEditionisacompilationandFreeze-Dryingofrobust,reproducibletechniquesfort
3、heconservationofawiderangeofbiologicalandFreeze-Dryingmaterials.Thiswidelyexpandedsecondeditionincludesnovelapproachesandprotocolsforbiologicalmaterialsthatwerenotpreservablewhenthefirsteditionwaspublishedin1995.Thisvolumebeginswithadiscussionoflongtermexsituconservationofbiolog-icalresources,therol
4、eofbiologicalresourcecenters,andfundamentalprinciplesofProtocolsProtocolsfreeze-dryingandcryopreservation.Eachchapterfocusesonthepreservationofspecificbiologicalmaterials,includingproteins,mircroorganisms,celllines,andmulticellularstructures.Detaileddescriptionsofmaterialsrequiredandstepwisepro-toco
5、lsarepresentedforeachpreservationmethod,alongwithexplanatorynotesthathighlightkeytechnicalissues.ThetroubleshootingandbackgroundnotesprovidedforeachprotocolenhancethispracticalandreliableresourcefortheconservationandSSECONDECONDEEDITIONDITIONsustainableuseofbiologicalresources.FEATURES•Backgroundand
6、Explanatorynotesforeach•Methodsforlyophilizationofproteinsprotocol•Newandnoveltechniquesforpreserving•Cutting-edgemethodsforconservationandbiologicalmaterialsEditedbyEditedbysustainableuseofresourcesCONTENTSSSLong-TermExSituConservationofBiologicalpreservationofFishSperm.CryopreservationofECONDJJohn
7、G.DayohnG.DayResourcesandtheRoleofBiologicalResourceAvianSpermatozoa.CryopreservationofAnimalCenters.ThePrinciplesofFreeze-Drying.PrinciplesandHumanCellLines.CryopreservationofPrimaryofCryopreservatio
