小鼠sil13rα2基因克隆与在毕赤酵母菌中的表达

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1、-------独创性声明秉承学校严谨的学风与优良的科学道德，本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，不包含本人或他人已申请学位或其他用途使用过的成果。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了致谢。申请学位论文与资料若有不实之处，本人承担一切相关责任。论文作者签名：日期：保护知识产权声明本人完全了解第四军医大学有关保护知识产权的规定，即：研究生在校攻读学位期间论文工作的知识产权单位属第四军医大学。

2、本人保证毕业离校后，发表论文或使用论文工作成果时署名单位仍然为第四军医大学。学校可以公布论文的全部或部分内容（含电子版，保密内容除外），可以采用影印，缩印或其他复制手段保存论文。学校有权允许论文被查阅和借阅，并在校园网上提供论文内容的浏览和下载服务。论文作者签名：导师签名：日期：-----------小鼠sIL-13Rα2基因克隆及在毕赤酵母菌中的表达研究生：蔡累学科专业：内科学（呼吸系病）所在单位：第四军医大学西京医院呼吸内科导师：李志奎教授（主任医师）辅导教师：资助基金项目：国家自然科学基金资助项目（NO:30770926）关键词：sIL-13R

3、α2；基因；克隆；表达；毕赤酵母菌中国人民解放军第四军医大学2009年05月西安-----------第四军医大学硕士学位论文目录缩略语表···················································································································1中文摘要·························································································

4、··························3英文摘要···················································································································5前言···················································································································7文献回顾·········

5、··········································································································9一IL-13与哮喘···························································································9二IL-13受体研究进展··························································

6、···················12三毕赤酵母表达系统···············································································20正文小鼠sIL-13Rα2基因的克隆及在毕赤酵母菌中的表达·············26实验一小鼠sIL-13Rα2基因的克隆························································271实验材料·········································

7、······················································272实验方法·······························································································293实验结果·······························································································34-----------4讨论····

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