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1、IL11保护中子照射后肠上皮损伤的Jak/STAT信号转导机制研究(作者:单位:邮编:)作者：王瑞娟，彭瑞云，高亚兵,常公民，徐新萍，付凯飞,罗庆良【摘要】目的：探讨IL"对中子照射后肠上皮内Jak/STAT通路的影响，并观察其对Bax和Bel2表达的影响。方法：BALB/c小鼠和IEC6细胞经4Gy中子照射和IL11处理分别作为肠上皮损伤的体内外模型，采用HE染色、Westernblot、EMSA、免疫组化和图像分析技术分别检测肠道病理变化、Jak1和STAT3活性及Bax和Bel2的表达。结果:⑴中子照射后，小鼠肠道损伤极为严重，且未
2、见明显再生；IL"治疗组隐窝上皮细胞及存活隐窝数量较多。(2)中子照射后，IEC6细胞Jak1和STAT3活性均减弱，IL11处理可增加二者活性。(3)中子照射后，小鼠肠道Bax表达增加,Bel2表达无明显改变；应用IL11可降低Bax表达，增加Bel2表达。结论：在中子辐射时，IL11可通过激活Jak/STAT通路,并下调Bax表达，上调Bel2表达，对肠上皮起到一定的保护作用。【关键词】放射；肠上皮；IL11;Jak/STAT;凋亡[Abstract]AIM:ToexploretheeffectofIL11ontheactivatio
3、nofJak/STATpathwayandtheexpressionsofBaxandBel2intheintestinalepithelialcellsexposedtoneutronradiation.METHODS:TheBALB/cmiceandIEC6,irradiatedby4GyneutronwithorwithoutIL11treatment,servedasinvivoandinvitromodelseperately.Thechangesoftheintestines,activityofJak1andSTAT3and
4、expressionsofBaxandBel2wereobservedbyHEstaining,Westernblot,EMSA,immunohistochemistryandimageanalysis.RESULTS:(1)Miceexposedtoneutronradiationshowedsevereintestinaldamagesandnoobviousregenerationwasseen.IL11treatedmicehadalargernumberofcryptalepithelialcellsandcrypts.(2)N
5、eutronradiationdecreasedtheactivitiesofJak1andSTAT3,whileIL11increasedtheiractivities・(3)NeutronradiationdecreasedtheexpressionofBaxanddidn'tchangethelevelofBel2inthemurineintestine.IL11administrationdecreasedtheexpressionofBaxandincreasedthatofBel2.CONCLUSION:Themechanis
6、moftheintestinalprotectionofIL11inneutronirradiationmightbethatIL11stimulationtriggeredactivationofJak/STATpathway,downregulatedtheexpressionofBaxandupregulatedtheexpressionofBel2.[Keywords]radiation;intestinalepithelium;interleukin11;【关键词】放射；肠上皮；IL11;Jak/STAT;凋亡[Abstract
7、]AIM:ToexploretheeffectofIL11ontheactivationofJak/STATpathwayandtheexpressionsofBaxandBel2intheintestinalepithelialcellsexposedtoneutronradiation.METHODS:TheBALB/cmiceandIEC6,irradiatedby4GyneutronwithorwithoutIL11treatment,servedasinvivoandinvitromodelseperately.Thechang
8、esoftheintestines,activityofJak1andSTAT3andexpressionsofBaxandBel2wereobservedbyHEstaining,Weste
