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1、GSTM1和CYP2D6基因多态性与肺癌敏感性的关系@中国公共卫生1999年第15卷第6期8一7.GSTM1和CYP2D6基因多态性与肺癌敏感性的关系广J州市卫生防疫站(广州5]0080)—塑中山医科大学卫生学院张桥——————～提要采用病例一对照研究方法.应用PCR技术检测59例肺癌患者,59例住院对照和73例健康对照的GSTM1(+)型频率.分别为57.6,49.2和49.3.差异无显着性;经病理分型后一在腺癌中GSTM](一)的频率(76.9)高出2十对照组(平均49.2)3.42～3.45倍.差异有显着性(P一0.015～0.01
2、7).提示GSTMI(一)型可能是肺腺癌的敏感性标记.瘟用PCR—RF[P技术检测肺癌组和住院对照组的CYP2D6Ch(TⅢ/T)基因型频率,分别为35.6和47.5.差异无显着性;经吸烟指效分层后在不吸烟者中,肺癌组的TⅢ/T型频率(19)显着低于住院对照组(47.1),OR一378.P一0.036,提示在不吸烟者中T…/T型可能是肺癌的一十保护因子.联合分析来见GSTM1和CYP2D6Ch有协同关系.关键词肺癌GSTMICYP2D6遗停多态性病例一对照研究～～StudyonthePolymorphismsofGSTM1andCYP2D
3、6genesas—sociatedwithsusceptibilitytolungcancerinChineseGaoYang,etatMunicipalHealthandAnti—EpidemicStationofGuangzhou(Guangzhou510080)Thecase—controlstudywasconductedtoexaminetheas--sociationbetweenGSTM1nullandCYP2D6Ch(TⅢ/T)geno—typesandlungcancerriskamongChineseofFlannat
4、ionalityIMnginGuangdong.GSTM1(一)wasdetectedwithpolymerasechainreaefion(PCR)inall191suhjeets,involving59lungcan—cercases.59hospitalcontrolsand73healthycontrols.ThefrequenciesofGSTM1(一)werenotsignificantdifferencebe—tweenthecasesandthetwocontrolsoveral1.However.amongadenoca
5、rcinomaoflung,thefrequencyofGSTM1(一)(76.9)washigherthanthatincontrols(49.2),andtheoddradioswere3.42…345TheresultssuggestedanelevatedriskforadenoeardnomaofLungwithGSTM](一).Polymerasechainreaction—restrictionfragmentlengthpolymorphisms(PCR—RFLP)wasusedtodetectCYP2D6Ch(T】/T)
6、genotypein59lungcancerpatientsand59hospitalcontrols,andno[slgnifi—cantdifferencebetweenthetwogroups.However,nonsmokerswithnonT日/T(c8E/CorCL8日/T)genotypeshad3.78foldincreasedriskoflungcancercomparedtothosewithT】日8/Tgenotype(withP一0.036).Noresultsuggestedasubstantialinterac
7、tioneffectbetweenGSTM1andCYP2D6polymor—phismsandtheriskoflungcancer.KeywordsLungNeoplasmGSTM]CYP2D6GeneticPolymorphismACase—ControlStudy致肺癌因素多为外源性化学致癌物.大多数外源性化学致癌物需经代谢酶催化后,与DNA等生物大分子结台并启动致癌过程.许多代谢酶存在遗传多态现象,研究表明某些代谢酶的遗传多态现象与肺癌敏感性有关,有可能做为肺癌的遗传标72记用于敏感性和危险度评价.细胞色素P4502D6(cyt
8、ochromesP4502D6.CYP2D6)和答胱昔肽S一转移酶M1型(glutathionS—transferaseM1,GSTM1)分属于I(恬性),I(解毒)相代谢酶.近年来研究表明.
