肠道菌群紊乱和自闭症4.doc

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1、肠道菌群紊乱和自闭症4Gut Pathog. 2013Apr12;5(1):9.doi:10.1186/1757-4749-5-9.Theneurotoxiceffectofclindamycin-induced gut bacterialimbalanceandorallyadministeredpropionicacidonDNAdamageassessedbythecometassay:protectivepotencyofcarnosineandcarnitine.El-AnsaryA1, ShakerGH, El-Gezeery

2、AR, Al-AyadhiL.Authorinformation·1DepartmentofBiochemistry,CollegeofScience,KingSaudUniversity,P,OBox22452,Riyadh11495,SaudiArabia.elansary@ksu.edu.sa.AbstractBACKGROUND:CometassayisaquickmethodforassessingDNAdamageinindividualcells.ItallowsthedetectionofsingleanddoubleDN

3、Astrandbreaks,whichrepresentthedirecteffectofsomedamagingagents.Thisstudyusesstandardcometquantificationmodelstocomparetheneurotoxiceffectoforallyadministeredpropionicacid(PA)tothatproducedasametaboliteofbacterialovergrowthinducedbyclindamycin.Additionally,theprotectiveef

4、fectofcarnosineandcarnitineasnaturaldietarysupplementsisassessed.METHODS:Singlecellgelelectrophoresis(cometassays)wereperformedonbraincortexandmedullasamplesafterremovalfromninegroupsofhamstersincluding:acontrol(untreated)group;PA-intoxicatedgroup;clindamycintreatedgroup;

5、clindamycin-carnosinegroupand;clindamycin-carnitinegroup.RESULTS:Thereweresignificantdoublestrandbreaksrecordedastaillength,tailmomentand%DNAdamageinPAandclindamycin-treatedgroupsforthecortexandmedullacomparedtothecontrolgroup.Neuroprotectiveeffectsofcarnosineandcarnitine

6、wereobserved.ReceiverOperatingCharacteristicscurve(ROC)analysisshowedsatisfactoryvaluesofsensitivityandspecificityofthecometassayparameters.CONCLUSION:PercentageDNAdamage,taillength,andtailmomentareadequatebiomarkersofPAneurotoxicityduetooraladministrationorasametaboliteo

7、finducedentericbacterialovergrowth.Establishingbiomarkersofthesetwoexposuresisimportantforprotectingchildren'shealthbydocumentingtheroleoftheimbalancein gut microbiota intheetiologyof autism throughthe gut-brainaxis.Theseoutcomeswillhelpeffortsdirectedatcontrollingtheprev

8、alenceof autism,a disorder recentlyrelatedtoPAneurotoxicity.DigDisSci. 2012Aug;57(8):1987-9.doi: