孕妇外周血中胎儿dna水平的检测用于诊断先兆早产

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1、孕妇外周血中胎儿DNA水平的检测用于诊断先兆早产980Clinica1MedicalJournalofChina,2006.Vo1.13,No.6中国临库廛孝2006年]2月第13卷第6期9■论着？孕妇外周血中胎儿DNA水平的检测用于诊断先兆早产王雪梅陶红纪向虹摘要目的:探讨孕妇外周血中胎儿DNA水平的检测在先兆早产诊断中的应用价值•方法:选择5()例先兆早产孕妇，按转归分为治疗无效的先兆早产组(30例)，治疗成功的先兆早产组(20例)，于有先兆早产征象时抽取外周血，另选5()倒正常孕妇作为对照组(B超确定3组孕妇所妊娠的胎儿均为男性),对应治疗无效，治疗成功的先兆早产组的孕周时间抽取外周血

2、.标本采用荧光定量PCR检测胎儿Y染色体上的性别决定基因(SRY基因)DNA水平，使用阴道超声诊断仪测量宫颈长度•结果:⑴治疗无效的先兆早产组胎儿DNA水平为(625.5±52.4)copy/ml;治疗成功的先兆早产组胎儿DNA水平为(220.4±57.5)copy/m[;对照组胎儿DNA水平为(189.2±43,2)copy/ml,治疗成功的先兆早产组较对照组明显升高，治疗无效的先兆早产组较治疗成功的先兆早产组明显升高，两者均有显着差异(P<()・(l).(2)治疗无效的先兆早产组平均宫颈长度为(28.9±6.8)mm,治疗成功的先兆早产组平均宫颈长度为(41.8±6.4mm),对照

3、组平均宫颈长度为(42.2±5.8)mm.治疗无效的先兆早产组与治疗成功的先兆早产组比较,P<0.01;治疗无效的先兆早产组与对照组比较有显着差异(P<0.01);对照组与治疗成功的先兆早产组比较无显着差异(P>0.05).结论:孕妇外周血胎儿DNA水平变化可作为预测先兆早产一个有应用价值的指标.关键词DNA;先兆早产;聚合酶链反应;官颈长度中图分类号R714.21文献标识码ARoleofMeasuringtheConcentrationofCirculatingNon-hostfetalDNAofPregnantWomenPlasnminPrenm-tureDeliver

4、yWANGXuemeiTAOHongJIXianghongDepartmentofObstetrics,QingdaoMunici加ZHospitalinShandongProvince,Qingdao266071AbstractObjective:ToinvestigatetherelationshipbetweentheconcentrationoffetalDNAinpregnantwomenplasmaandprematuredelivery.Methods:50casesconsistingof30prematuredeliverywomenand20threatenedprema

5、turedeliveryandan一otherSOcasesofnormalpregnancywereselectedtoqualifySRYlevelsinmaternalbloodwithafluorescentquantitativepoly一merasechainreaction(QF_PCR)andmeasuretheconcentrationoffetalDNAwithSRYgene.ThegestationalagesofthetwogroupshavenosignificantdifferenceandallfetusweremaledetectedbyBultrasonic

6、lhecervixlengthwasmeasuredwithtrans一vaginalsonography.Results:(l)thefetalDNAconcentrationof30prematurede!iverycasesis625.54±52.4copy/ml:whiletheother20threatenedprematuredeliverycasesis220.4土57.5copy/ml.thecontrolgroupis189.244-43.2copy/rn1.Theva一fianceanalysisandimpairQtestindicatethatthefeta1DNA1

7、evelinmatema1bloodoftheprematuredeliveryishigherthanthethreatenedprematuredelivery.anditisalsohigherinthreatenedprematuredeliverygroupthaninthenormalgroup.Boththedifferencesaresignificant(P<O・OOl)・(2)The