大鼠骨髓间充质干细胞体外诱导分化为肝细胞

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1、泸州医学院学报2010年第33卷第l期JournalofLuzhouMedicalCoUegeVo1．33No．12010DIFFEIENTIATIONOFMARROWBONEMESENCHYMALSTEMCELLSINTOHEPATOCYTESFROMRATINVITROWangZhongqiong，LvMuhan，XiaShiping，ZhongXiaolin，LiChangpingDepartmentofGastroenterology，theAfiliatedHospitalofLuzhouMedicalColle

2、ge，Luzhou，646000，chinaAbstractObjectives：Tostudythepotentialofhepatocytegrowthfactor(HGF)，acidfibroblastgrowthfactorfaFGF)andoncostatinM(OSM)toinduceMSCsdifferentiateintohepatocytesandtheirroles．Methods：MSCsweretreatedwithHGF，aFGFandOSM．DetectingtheexpressionofAFP

3、，ALBandureawasperformedbyehe—mi—luminescenttechnique，RT-PCRmethodandglutamicaciddehydrogenasetechnique．Results：AFPexpressionemergedineverygroupexceptcontrolgrouprPl

4、OSMandaFGFcaninduceMSCsdifferentiateintohepatocyteswiththeexpressionofAFP，ALBandexcretingurea，whileaFGFandOSMcannotinduceMSCsdif-ferentiateintomaturehepatocytewithoutHGF．KeywordsBonemarrowmesenchymalstemcells；Hepatocytegrowthfactor；Acidicfibroblastgrowthfactors；On

5、costatinM：HepatocytesCLCNamberR734．2DocumentCodeAArticleID10O0—2669(2010)1-0040-05大鼠骨髓间充质干细胞体外诱导分化为肝细胞王忠琼，夏世萍，刘彦，钟晓琳，李昌平(泸州医学院附属医院消化内科，四川1泸外I646000)摘要目的：研究HGF、aFGF及OSM诱导MSCs成为肝细胞的能力及其在分化中的作用。方法：用HGF、aFGF、OSM诱导分化MSCs后，用化学发光法、R．T—PCP．法及谷氨酸脱氢酶法检测细胞AFP、ALB及尿素的表达情况。结果：

6、除对照组外其余各组细胞均有AFP表达(Pl

7、lrejectionwhichhasaeffectivetherapeuticmeasureforliverfailure，butthisgreatimpactonitsclinicalapplication【．Accord-therapyisfaeedwithhepaticsourceshortage，ingly，searchingfororiginalhep—atocellularsourceisimmunotoxicityandexpensivetreatment．Hepatocytedebatedintherese

8、archareahotly．Marrowbonemese-transplantation，(HCT)isoneofthemethodswhichnchymalstemcells(MSCs)candifferentiateintobone，replacethewholelivertransplantati