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大小:8.02 MB
页数:82页
时间:2018-12-09
《crisprcas9技术在制备牛myostatin基因敲除细胞株上的应》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、优秀毕业论文ClassifiedIndex:Code:10224Confidential(yes/no):NoNO.:130920863DissertationfortheProfessionalMasterDegreeApplicationOfCRISPR/Cas9ForMyostatinGeneKnockoutCellLinesCandidate:YangYuSupervisor:YanYunqinChenHongyanDegreeCategory:MasterofEngineeringColleg
2、e:CollegeofLifeScienceResearchField:BiologyStudyMode:BioengineeringHarbinChinaJune2015精品参考文献资料优秀毕业论文万方数据精品参考文献资料优秀毕业论文目录lYIIIIi21111171111117lllll2lllll2IH2IIllilll摘要一iI一矗_:_-.:I精品参考文献资料优秀毕业论文万方数据精品参考文献资料优秀毕业论文东北农业大学工程硕士学位论文4讨论..234.1cRIsP刚Cas9技术的选择.234.
3、2MSTN基因靶位点的选择.234.3转染方法的选择244.4细胞单克隆培养的注意事项245结{仑..25致{射一26参考文献.27攻读硕士学位期间发表的学术论文31精品参考文献资料优秀毕业论文万方数据精品参考文献资料优秀毕业论文CONlENTSCONTENTSAbstract.................................................................IAbstractinEnglish.。.。,,..。。。...。.。。。........。....
4、......。。。。。。....。......。.。。..。...。。.IllIIntroduction....................................................................................11.1ResearchprogressofCRISPR/Cas9technology....11.1.1GenerationanddevelopmentCRISPR/Cas9technology.11。1.2Thestructureo
5、ftheCRISPR/Cassystem.........................................11.1。3TheactionmechanismoftypeIICRISPR./Cassystems.............................21.1.4PrecautionsofdesigningtargetsitesbyCRISPR/Cas9technology..................21.1.5ApplicationofCRISPR/Cas9tech
6、nology.....3i.2Thepurposeandsignificanceofthestudy.......................····.··············-·”41.2.1ThestructureandfunctionofMSTNgene41.2.2ResearchsurveyofMSTNgene...................................-.·-...-41。3Thepurposeandsignificanceofthestudy.52Mater
7、ialsandMethods.62.1experimentalmaterials...62.1.1Strain.plasmidandcell.62.1.2Enzyme.kitandotheragents............................................-······-·62.1.3Majorinstrument........62.1.4Analysissoftware.--.72.1.5Preparationformajoragents·一·72.2Content
8、andmethods..............................................................···....82.2.1ConstructionofCRISPR/Cas9targetingvector...............,............................-..。。,82.2.2Cellcryopreservationandrecovery..........
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