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ID:78625932
大小:792.50 KB
页数:14页
时间:2022-02-04
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1、EfficientandstablerescueofclassicalswinefevervirusfromclonedcDNAusinganRNApolymeraseIIsystemWangLuNovember1.Background2.Work3.Discussion1.BackgroundReversegeneticsisamethodforstudyingtheproteinfunctionsofRNAviruses.ThreemethodsforrescuingvirusesThefirstisbasedoninvitrotranscriptionofinfect
2、iousRNAThesecondisbasedonthetranscriptionofinfectiousRNAwithincellsThethirdisbasedoninfectiouscDNAclones.ResultsCellularRNApolymeraseIIissuperiortoT7RNApolymeraseforrapidandefficientvirusrescue.thecytomegalovirus(CMV)promoterandT7promotertoenablerescuebyeitherofthemethodsdescribedabove.rib
3、ozyme(HamRz)andhepatitisdeltavirusribozyme(HDVRz)ensureintegralandprecisetranscriptionofthegenomeThisefficient,convenientandeconomicalmethodofvirusrescuewillbeusefulforrescuingattenuatedvirusesBesides,Genetictag(AvaⅠ)intheviruseswereanalyzed(Notshown)
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