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1、中国医科大学学报JChinMedUniv第34卷第4期2005年8月·301·高糖、糖基化终产物对大鼠主动脉平滑肌细胞巨噬细胞炎性蛋白1α表达的影响1213孟馨,商秀丽,张锦,刘中华(1.中国医科大学附属第一医院内分泌科,辽宁沈阳110001;2.附属第一医院神经内科;3.沈阳军区司令部门诊部)【摘要】目的:探讨高糖浓度和糖基化终产物(AGEs)对大鼠主动脉平滑肌细胞巨噬细胞炎性蛋白(MIP)1αmRNA及蛋白表达的影响。方法:将培养的大鼠主动脉平滑肌细胞分别用不同浓度AGEs(100,200,400mg/L)单独培养2
2、4h;400mg/LAGEs和22mmol/L葡萄糖联合培养24h。RT2PCR方法及流式细胞仪检测MIP21αmRNA及蛋白的表达。结果:对照组平滑肌细胞内MIP21α呈弱表达;100,200,400mg/LAGEs培养平滑肌细胞24h及22mmol/L葡萄糖和400mg/LAGEs联合孵育24h后显著增高MIP21αmRNA的表达,其电泳条带相对积分吸光度值分别为对照组的1.36,1.75,2.45及2.76倍(P<0.05);各组平滑肌细胞MIP21α蛋白的平均荧光强度分别为对照组的1.24,1.63,2.37及2
3、.68倍(P<0.05)。结论:AGEs能刺激平滑肌细胞MIP21αmRNA及蛋白表达增加,且与高糖具有协同作用。【关键词】葡萄糖;糖基化终产物;巨噬细胞炎性蛋白1α;平滑肌细胞,血管;大鼠【中图分类号】Q49314【文献标识码】A【文章编号】0258-4646(2005)04-0301-03EffectofhighconcentrationofglucoseandAGEsonthemRNAandproteinexpressionofmacrophageinflammatoryprotein21αinculturedVS
4、MCs1213MENGXin,SHANGXiu2li,ZHANGJin,LIUZhong2hua(1.DepartmentofEndocrinology,TheFirstAffiliatedHospital,ChinaMedicalUniversity,Shenyang110001,China;2.DepartmentofNeurologyTheFirstAffiliatedHospital;3.OutpatientDepartment,CommandofShenyangMilitaryArea)【Abstract】Ob
5、jective:Toinvestigatetheeffectofhighconcentrationofglucoseandadvancedglycationendprod2ucts(AGEs)onthemRNAandproteinexpressionofmacrophageinflammatoryprotein21α(MIP21α)inculturedvascularsmoothmusclecells(VSMCs).Methods:VSMCswereculturedwithdifferentconcentrationso
6、fACEs(100,200,and400mg/L)for24hoursandcoculturedwith22mmol/Lofglucoseand400mg/LofAGEsfor24hours.ThelevelsofmR2NAandproteinexpressionofMIP21αinculturedVSMCsweredetectedbyRT2PCRandflowcytometerrespectively.Re2sults:ThemRNAexpressionofMIP21αincreased1.36fold,1.75fol
7、d,2.45fold,and2.76fold,andtheproteinexpres2sionofMIP21αincreased1.24fold,1.63fold,2.37fold,and2.68foldinVSMCsculturedwithdifferentconcentrationsofAGEsandcoculturedwithglucoseandAGEscomparedwiththeBSAgroup(P<0.05).Theaveragefluorescentinten2sityvaluesofMIP21αwere1
8、97±3,260±3,375±6,and423±8inVSMCsand158±4inBSAgroup.Conclusion:AGEscouldinduceahighmRNAandproteinexpressionofMIP21αinculturedVSCMsinadose2dependentmanner.Therem
